Expression of integrin α6A and α6B mRNA splice variants in adult mouse BM and FL HSPCs. Adult mouse BM and E14.5 FL LSK and Lin⁻Sca-1⁻c-Kit⁺ cells were gated for sorting (left panels). The vertical and horizontal bars were set on the basis of isotype-matched negative control profiles (99.3% of cells negative). Reanalysis of each cell population showed more than 99% purity. RT-PCR analysis for integrin α6A and α6B mRNA splice variants of FL and BM LSK and Lin⁻Sca-1⁻c-Kit⁺ cells is shown in the right panel. MW indicates positions of 500-bp and 400-bp molecular weight markers on the gel. The bands corresponding to 510-bp and 378-bp integrin α6A and α6B splice variants are seen in all 4 cell populations. PCR for GAPDH shows the relative amounts of mRNA in the corresponding samples. Shown is 1 representative of 2 experiments.