Adhesion of mouse fetal liver LSK cells to laminins and inhibition of cell adhesion by antibodies against integrin α6 and β1 chains. (A) The adhesion of integrin α6^−/− FL LSK cells (α6^−/−) and wild-type littermates (α6^+/+) to laminin-411 (LM-411) (30 μg/mL), laminin-511 (LM-511) (10 μg/mL), and laminin-511/521 (LM-511/521) (10 μg/mL). PBS indicates adhesion to wells coated with PBS instead of proteins; FN, adhesion to wells coated with fibronectin. Data represent mean (± SEM) from 2 experiments performed in triplicate. Asterisks indicate differences compared with wild-type cells. (B-D) Adhesion of LSK cells to wells coated with LM-411 (B), LM-511 (C), and LM-511/521 (D) in the presence of antibodies GoH3 against integrin α6 chain (α6), Ha2/5 against integrin β1 chain (β1), or control rat or hamster monoclonal antibodies (rIgG and hIgM, respectively). PBS indicates cell adhesion to wells coated with PBS instead of proteins. The results are shown as percentage adherent cells of mean adhesion obtained after treatment with rIgG control antibody. Data are mean (± SEM) from 2 to 3 experiments performed in triplicate.