Figure 5
![Figure 5. Impact of mutations in the C-terminal tyrosines of LAT on PI3K and PLC activation in response to GPVI triggering. [32P]-labeled platelets from WT (♦) and Lat3YF (■) (A) or from WT (Rag−/−) (♦) and LatY136F mice (●) (B) platelets were stimulated by 5 nM Cvx for the indicated times and the levels of [32P]-PtdIns(3,4,5)P3, [32P]-PtdIns(3,4)P2, [32P]-PtdIns(4,5)P2, and [32P]-PtdOH were analyzed as indicated in “Platelet preparation and in vitro aggregation studies.” Results are means (± SEM) of 4 experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/110/7/10.1182_blood-2007-02-075432/2/zh80190707710005.jpeg?Expires=1724237924&Signature=lv3tQK105vqIKAniotvB0hnJKwBTc5-PBnfJ7Hst9PRt4F3dy-Z~~nk2493NpzQCdgxadir6VHDy~bSQC3JXlEdsWEF9RG1VNDmt2BbSybXAbCmyv0FUMNb9M4DA~rOgcM937KFF3Tp8Zs3wuINJ8WU2L2SyDOntvWM0on6Kautdj60~bvu4dHcBNNgQs-tQj0GpL51Gvb710iQ0zh1vGKNpovqYHk-1jSGL1YVlC0oHPd9sr7JRUpmjgtYO~aNyQjf1FB85wv5HxQbsVzPJgmjER6~fMBeE8NKJYVkx2Kpx-EQWdfaXDEGZ~LTNMjVaJaiMQ8fGrKlCSbGn1xUKBw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Impact of mutations in the C-terminal tyrosines of LAT on PI3K and PLC activation in response to GPVI triggering. [32P]-labeled platelets from WT (♦) and Lat3YF (■) (A) or from WT (Rag−/−) (♦) and LatY136F mice (●) (B) platelets were stimulated by 5 nM Cvx for the indicated times and the levels of [32P]-PtdIns(3,4,5)P3, [32P]-PtdIns(3,4)P2, [32P]-PtdIns(4,5)P2, and [32P]-PtdOH were analyzed as indicated in “Platelet preparation and in vitro aggregation studies.” Results are means (± SEM) of 4 experiments.
