In vivo functional consequences of HS1 deficiency in mice. (A) The mice's tails were clipped 0.3 cm from the end and submerged in 0.9% saline. The clipped portions were isolated and subjected to genotyping of the HS1 locus by PCR. (B) Time until the stoppage of bleeding was measured; the times were then compared with that of the PCR analysis (n > 15). Statistical analysis by ANOVA revealed a P value less than .01 between HS1-deficient and wild-type (WT) mice and HS1-deficient and HS1-heterozygote (HET) mice. Error bars indicate standard error. (C-E) Wild-type (n = 8) (C) and HS1- (n = 11) (D) and P2Y1- (n = 8) (E) deficient mice were injured by 10% FeCl₃ for 2 minutes, and time to occlusion (TTO) was measured by optical Doppler flow. (F) Time to 90% occlusion was calculated and graphed. Statistical analysis by t test revealed a P value less than .01 between wild-type and HS1-deficient mice and wild-type and P2Y1-deficient mice. *Statistically significant (P < .01).