Figure 4
Figure 4. FANCJ-RPA interaction is intact in FA mutant cells coimmunoprecipitation of FANCJ and RPA in FA-A and FA-D2 cells. Coimmunoprecipitation of RPA with FANCJ in FA-A vector and FA-A corrected cells (A) or FA-D2 vector and FA-D2–corrected cells (B) with the use of FANCJ antibody. The blot was probed with rabbit anti-FANCJ (top) and mouse anti-RPA (bottom) antibodies. Input represents 15% of WCE input for coimmunoprecipitation experiments. Control immunoprecipitate from WCE of FA-A–corrected or FA-D2–corrected cells with the use of normal rabbit IgG is shown in lane 5.

FANCJ-RPA interaction is intact in FA mutant cells coimmunoprecipitation of FANCJ and RPA in FA-A and FA-D2 cells. Coimmunoprecipitation of RPA with FANCJ in FA-A vector and FA-A corrected cells (A) or FA-D2 vector and FA-D2–corrected cells (B) with the use of FANCJ antibody. The blot was probed with rabbit anti-FANCJ (top) and mouse anti-RPA (bottom) antibodies. Input represents 15% of WCE input for coimmunoprecipitation experiments. Control immunoprecipitate from WCE of FA-A–corrected or FA-D2–corrected cells with the use of normal rabbit IgG is shown in lane 5.

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