Coimmunoprecipitation of FANCJ and RPA after DNA damage. Nuclear extracts were prepared from HeLa cells that were untreated or exposed to IR (10 Gy) or MMC (500 ng/mL) and immunoprecipitated with anti-FANCJ or anti-RPA antibody as indicated. The blot was probed with rabbit anti-FANCJ (top) and mouse anti-RPA70 or anti-RPA32 (bottom) antibodies.