EMPs are involved in plasmin generation in matrigel. (A) Plasmin generation in matrigel is dependent on the amount of EMPs. The chromogenic substrate CBS0065 was added to the matrigel with the EMPs, and under these conditions an increase in plasmin formation was detected from 105 EMPs (P < .001) after 2 hours of incubation. Asterisks indicate significant changes (P < .05). Results are the mean (± SD) of 3 independent experiments. (B) Plasmin generation in matrigel at different EMP amounts is dependent on the supply of plasminogen by FCS. The graph shows plasmin activity detected after 3 hours of incubation ▒, medium with 10% FCS; ▓, medium with plasminogen-free FCS; ▤, medium with plasminogen-free FCS supplemented with 1 μM plasminogen). Activity in the absence of EMPs may represent low levels of plasmin and/or limited amounts of activators in matrigel. Asterisks indicate significant changes (P < .05). Results are the mean (± SD) of 3 independent experiments.
