NPI-0052 represses TNF-induced NF-κB–dependent expression of proliferation-, antiapoptosis-, and metastasis-related gene products. Proliferative (A), antiapoptotic (B), and metastatic (C) gene products are shown. KBM-5 cells were incubated with 50 nM NPI-0052 for 4 hours and then treated with 1 nM TNF for the indicated times. Whole-cell extracts were prepared, and 30 μg of the whole-cell lysate was resolved by SDS-PAGE, electrotransferred to nitrocellulose membrane, sliced from the membrane based on the molecular weight, and then probed with antibodies against survivin, IAP1/2, Bcl-2, Bcl-x_L, cFLIP, TRAF1, VEGF, MMP-9, ICAM-1, COX-2, c-Myc, Cyclin D1, or β-actin as described in “Materials and methods.” TNF-treated and TNF plus NPI-0052-treated samples were run on the same gel under identical conditions and probed with the same immunoblotting solutions.