Sequence traces and restriction enzyme-based assessment of the new JAK2 mutation. (A) Electropherogram showing the c.51324–51329 deletion in exon 12 of the JAK2 gene. The arrows indicate the position of deleted nucleotides. (B) DNA gel electrophoresis DNA of exon 12 including the deletion before (lanes 1, 3, and 5) and after (lanes 2, 4, and 6) digestion with MboII, derived from patients (lanes 1 trough 4) and control (lanes 5 and 6). MW indicates 50-bp ladder DNA molecular-weight markers; and *, the 195-bp fragment containing the deletion and lacking of a MboII site, which substitutes for 115-bp and 86-bp fragments (arrows).