Expression of Anxa2 protein in murine long bones. Mouse tibias were fixed in 10% formalin at 4°C before being decalcified in 10% EDTA (pH 7.4) and embedded in paraffin wax. Immunolocalization of Anxa2 protein (arrows) was visualized using a monoclonal antibody that was raised against Anxa2 or an IgG control antibody and a 9 ethyl-carbazole chromogen kit. Anxa2-immunostained tibias (20 × magnification) (A,C,E) and control immunoreactivity (B,D,F). Scale bars represent 50 μm. (A-B) Anxa2 expression at the epiphyseal growth plates. (C-D) Anxa2 expression in the diaphysis regions. (E-F) Anxa2 expression in bone marrow endothelial cells. (G-H) Higher magnification of panels C and E. Images at 20× (A-F) and 40× (G, H) via a 0.75 NA oil objective. A Nikon E800 (Diagnostic Instruments, Sterling Heights, MI) camera and Image-Pro Plus software (MediaCybernetics, Bethesda, MD) were used.