Cell blot–identified annexin II (Anxa2) expressed on osteoblasts (OBs) mediates adhesion of KG1a cells. SaOS-2 or MG-63 membrane lysates were run on native lithium dodecyl sulfate–polyacrylamide gels before being blotted onto polyvinylidene fluoride membranes.^20,21  After blocking the membranes with bovine serum albumin (BSA), the membranes were probed with biotin-labeled KGla cells for 2 hours at 37°C. The membranes were then fixed and the location of the cells identified with streptavidin-conjugated horseradish peroxidase. The figure is presented as an enhanced image for visualization. (The original figure is provided in Figure S1, available on the Blood website; see the Supplemental Figures link at the top of the online article.) Adhesion of the biotin-labeled KG1a cells to proteins in the MG-63 membrane lysates revealed a band at approximately 36 and approximately 94 kDa.