Expression of cell surface IL-2R, IL-7R, and IL-15R subunits and downstream signaling molecules in EBV-transformed B and ED-γc-7R cell lines. (A) EBV-γc^low, EBV-γc^null, EBV-γc^wt, and ED-γc-7R cells were labeled with either antibodies to IL-2Rγ, IL-2Rα, IL-2Rβ, IL-7Rα, or IL-15Rα (solid lines) or to the isotype control (gray filled) and subjected to FACS analysis. Percentages shown in each panel represent the proportion of cells expressing detectable levels of the indicated cell surface receptor subunit. (B) Lysates of the above cell lines were analyzed for Jak3, STAT5 or actin using SDS-PAGE and Western blotting. Immobilized proteins were detected using HRP-conjugated secondary reagents and enhanced chemilumiscence.