Surface phenotype of CD34+ progenitors after 3-week culture in 2% to 3% versus 20% O2 in osteoblastic-derived factors. CD34+ progenitors were plated on FN-coated plates in the presence of SCF, IL-6, and CXCL12 for 3 weeks, with half medium changes every 72 hours. (A) A representative phenotype of one experiment (above) and mean ± SEM (below) of 3 independent cord blood experiments comparing 20% (gray) versus 2% to 3% (black) O2. (B) Cells recovered from the 20% (gray) and 2% to 3% (black) O2 cultures were plated in methylcellulose medium with complete cytokines, and scored after 12 to 14 days. (C) CFU colonies were harvested and labeled with antibodies against CD34, GlyA, CD41, and CD61. FACS plot of representative example is shown (above), as well as mean ± SEM from 3 independent experiments (below).
