GILZ in APCs from GC-treated patients modulates the production of cytokines and chemokines and induces Tregs. APCs were collected before (■) and 48 hours after (▩) the initiation of GC treatment and they were nucleofected with siC or siGILZ. (A) APCs were stimulated with LPS and their production of CCL5, TNFα, and IL-10 was determined by ELISA (mean ± SEM). (B-D) APCs were pulsed with PPD and used to activate CD4⁺ T lymphocytes. (B) Expression of IL-10 by CFSE^lo CD4⁺ T lymphocytes was determined by flow cytometry. Control mAb staining obtained with APCs nucleofected with siC is shown as a dotted line. (C-D) CD4⁺ T lymphocytes were added in various numbers to CFSE-labeled PBMCs. Proliferation of CD4⁺ responders was assessed by flow cytometry. Means ± SEM from 3 experiments are shown in (A) and (C), and a representative experiment is shown in (B) and (D). CD4⁺ T lymphocytes (5 × 10⁴) were added to PBMCs in panel D. *P < .05 using a Wilcoxon test.