Antigen specificity of the Tregs induced by GILZ-transfected DCs. (A-B) Control and GILZ-transfected DCs were pulsed with either PPD or CMV (first stimulation) and used to stimulate CD4⁺ T lymphocytes, which were then added to CFSE-labeled PBMCs stimulated with either PPD or CMV (second stimulation). Proliferation of CFSE-labeled CD4⁺ responders (A) and CD8⁺ responders (B) was assessed by flow cytometry. Results are representative of 4 experiments.