GILZ-transfected DCs stimulate the expression of Treg markers by antigen-specific CD4⁺ T lymphocytes. Control and GILZ-transfected DCs were pulsed with PPD and used to stimulate CFSE-labeled CD4⁺ T lymphocytes. (A-B) Expression of Treg markers by CFSE^hi and CFSE^lo CD4⁺ T lymphocytes was determined. A typical of 3 independent experiments is shown. Control mAb staining obtained with GILZ-transfected DCs is shown as a dotted line in panel B. (C) Expression of IL-10 and FOXP3 by CFSE^lo CD4⁺ cells was determined. A typical example of 2 independent experiments is shown.