American Society of Hematology

Figure 1

$Figure 1. Expression of CD25hi and FOXP3 by CD4+ T lymphocytes stimulated with DEX-treated DCs. (A-B) DCs were treated with DEX, pulsed with PPD, and used to stimulate CD4+ T lymphocytes. The fraction of CD25hiFOXP3+ cells among CD4+ T lymphocytes was then determined. Results representative of 5 experiments with DEX (10−7 M) are shown in panel A, and means ± SEM of 3 experiments with various concentrations of DEX are shown in panel B. (C) DCs were treated with various concentrations of DEX and production of GILZ was analyzed by Western blot. Quantification of actin was used as an internal control. MW indicates molecular weight markers. One representative of 3 experiments is shown on the top of the panel. The ratio between the intensities of GILZ and actin expressions in 3 experiments (mean ± SEM) is shown on the bottom of the panel.$

Expression of CD25^hi and FOXP3 by CD4⁺ T lymphocytes stimulated with DEX-treated DCs. (A-B) DCs were treated with DEX, pulsed with PPD, and used to stimulate CD4⁺ T lymphocytes. The fraction of CD25^hiFOXP3⁺ cells among CD4⁺ T lymphocytes was then determined. Results representative of 5 experiments with DEX (10⁻⁷ M) are shown in panel A, and means ± SEM of 3 experiments with various concentrations of DEX are shown in panel B. (C) DCs were treated with various concentrations of DEX and production of GILZ was analyzed by Western blot. Quantification of actin was used as an internal control. MW indicates molecular weight markers. One representative of 3 experiments is shown on the top of the panel. The ratio between the intensities of GILZ and actin expressions in 3 experiments (mean ± SEM) is shown on the bottom of the panel.

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