Study design. Animals were mobilized with granulocyte colony–stimulating factor/SCF for 4 days and PBMCs collected by leukapheresis, followed by CD34+ selection. They underwent transplantation after TBI. We compared 3 groups: one group received KGF once before, the second 3 times before, and the third 3 times after conditioning. These animals were compared with a control group that underwent transplantation without KGF treatment. All were vaccinated with an HIV env vaccine 3.5 months after transplantation, and followed for 12 months after transplantation. They were euthanized and underwent necropsy to collect all lymphoid tissue at the end of study.