Detection of the fusion gene formed by the genes for RNA binding motif 6 (RBM6) and colony stimulating factor 1 receptor (CSF1R). (A) Schematic diagram shows the RBM6, CSF1R, and RBM6-CSF1R proteins. The position of the breakpoint is indicated by arrowhead. POZ indicates poxvirus and zinc finger domain; RRM1 and RRM2, RNA recognition motif 1 and 2, respectively; TM, transmembrane; JM, juxtamembrane; and NLS, nuclear localization signal. (B) RT-PCR reaction indicated a fusion of RBM6 to CSF1R in RNA from MKPL-1 cells. GAPDH was used as a control. The sequence from junction of the fusion gene is listed at the bottom. (C) Genomic PCR reaction detected the RBM6-CSF1R fusion gene in DNA.