BM c-Kit⁺Lin^low cells from Gab2^−/− mice are deficient in phosphorylation of S6 and ERK. Whole BM from wild-type and Gab2^−/− mice was serum and cytokine starved for 4 hours and stimulated with IL-3 (10 ng/mL) for 5, 15, or 30 minutes alone or in the presence of DMSO control (0.1%), 50 μM PD98059 (for pERK measurement), or 50 μM LY294002 (for pS6 measurement). Levels of phosphorylated ERK and S6 were measured using phosphospecific flow cytometry in the c-Kit⁺/Lin^−/low subset. Flow cytometry profiles are shown in the top panels for basal (starved and unstimulated) or IL-3 stimulated cells. Values of the median intensity of fluorescence for each phosphoprotein measured are provided below each set of flow cytometry histograms. Light-gray bars are wild-type, and dark-gray bars are Gab2^−/−. Shown is 1 representative experiment from 5 total experiments.