PAR2, and not PAR1, silencing attenuates FVIIa-mediated TF mobilization from the Golgi. Mock-transfected, or PAR1 or PAR2 siRNA–transfected cells were exposed to FVIIa (10 nM) or thrombin (10 nM) for 2 hours at 37°C. Thereafter, the cells were fixed, permeabilized, and immunostained for the TF and the golgin-97. Panel A depicts representative images of immunostaining and panel B shows quantified data (mean ± SEM from 3 to 4 experiments).