Inhibition of PAR2 impairs FVIIa-induced tissue factor mobilization. Fibroblasts were treated with a combination of PAR1 monoclonal antibodies (ATAP-2, 10 μg/mL; WEDE-15, 25 μg/mL) or rabbit antihuman PAR2 IgG (200 μg/mL) for 30 minutes at the room temperature. Then, the cells were exposed to FVIIa (10 nM) or thrombin (10 nM) for 2 hours at 37°C. Following the treatment, the cells were fixed, permeabilized, and immunostained for TF and the Golgi marker, golgin-97, as described in Figure 2. Panel A depicts representative images of immunostaining. Left panel images represent TF staining, middle panel images represent golgin-97 staining, and right panel images represent the overlay of TF and the Golgi marker staining (colocalization). Panel B shows percentage of total number of cells with TF pool intact in the Golgi (mean ± SEM from 3 to 4 experiments).