R18 disrupts interaction between 14–3-3 and FOXO3a but not 14–3-3–BAD association in Ba/F3 cells transformed by ZNF198-FGFR1. (A) 4ZF-transformed TonBaF cells inducibly expressing YFP-tagged R18 dimer or mutant were cultured with Dox in the absence of IL-3 for 24 and 48 hours. Coimmunoprecipitated FOXO3a, BAD, and YFP-tagged R18 dimer or mutant in 14–3-3 immunoprecipitates were detected by specific antibodies. (B) Phosphorylation levels of FOXO3a, BAD, PI3K, AKT, and ERK in the cell lysates were probed by Western blotting as controls.