Increasing CCN3 expression in Bcr-Abl⁺ cells causes accumulation in the sub-G₀ area of the cell cycle. (A) Protein lysates were extracted from K562 cells 24 hours after transfection with vector alone or vector containing the full-length CCN3 construct. Western blotting was performed to detect CCN3 protein. (B) K562 cells transfected with vector alone or vector containing the full-length CCN3 construct were harvested at 24 hours, fixed, and stained with propidium iodide. Cell-cycle profiles were generated and compared for cells containing the vector alone (i) and vector with CCN3 (ii).