Induction of EGFP in peripheral organs of SCL-tTA-2S/EGFP-lacZ double-transgenic mice is primarily restricted to hematopoietic cells and a subset of organ-specific cells in the kidney. Representative FACS profiles of collagenase-digested tissues from lung, heart, kidney, tongue, and esophagus are shown. Left panel (-DOX): Induced organs of bitransgenic mice do contain a small fraction of EGFP⁺ cells (bottom right quadrant). Percentages of EGFP-expressing cells are shown in the top right quadrant. Central panel: The EGFP⁺ fraction of cells from the left panel of organ plots (indicated by an arrow) was used for plotting CD45/TER119 pan-hematopoietic markers (y-axis) against the PECAM-1 endothelial marker (x-axis). Right panel: Percentages of EGFP⁺ hematopoietic cells are shown in the large top box and percentages of endothelial cells in the bottom right box. The percentage of EGFP-expressing cells lacking blood and endothelial markers is indicated in bottom box on the left. Note the substantial increase of EGFP-expressing double-negative CD45^-/TER119^-and PECAM-1^- cells in the kidney.