Production of specific Abs in culture. PBMCs from healthy controls, patients with CHI, and LTNP patients were stimulated with CpG ODN + IL-2 + IL10 + CD40 mAb. Cell culture supernatants were collected after 6 days of culture and used to measure anti-pneumococcus IgG and IgM, and anti-measles IgG and IgM Abs.