Figure 3.
Figure 3. T-bet negatively regulates cytokine-induced IL-17 production but does not augment cytokine receptor expression on Th1 or Th1β cells. (A) WT or Tbx21-/- Th1 or Th1β cells were cultured for 5 days as in Figure 1A (1° culture). Cells were restimulated with media alone or the indicated cytokines (2° culture) for 18 hours and analyzed for IFN-γ or IL-17 production using ELISA. ND indicates not detected. (B) WT or Tbx21-/- Th1 or Th1β cells were cultured as in Figure 1A. IL-12Rβ2, IL-23R, or IL-18Rα expression was assessed using flow cytometry. Numbers within each histogram represent mean fluorescent intensities averaged from 2 independent experiments. Black line indicates receptor staining; gray fill, control staining. ELISA data are represented as mean ± SE of replicate samples.

T-bet negatively regulates cytokine-induced IL-17 production but does not augment cytokine receptor expression on Th1 or Th1β cells. (A) WT or Tbx21-/- Th1 or Th1β cells were cultured for 5 days as in Figure 1A (1° culture). Cells were restimulated with media alone or the indicated cytokines (2° culture) for 18 hours and analyzed for IFN-γ or IL-17 production using ELISA. ND indicates not detected. (B) WT or Tbx21-/- Th1 or Th1β cells were cultured as in Figure 1A. IL-12Rβ2, IL-23R, or IL-18Rα expression was assessed using flow cytometry. Numbers within each histogram represent mean fluorescent intensities averaged from 2 independent experiments. Black line indicates receptor staining; gray fill, control staining. ELISA data are represented as mean ± SE of replicate samples.

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