VAP-1 supports rolling and firm adhesion of cord blood lymphocytes under physiologically relevant shear. VAP-1 was transfected into HUVECs, and the binding of cord blood lymphocytes was determined using the flow chamber assay under laminar shear stress of 1 dyn/cm.²  HUVECs were activated with TNF-α, and the numbers of the rolling cells (A), firmly adherent cells (B-C) (both from the rolling/firm adhesion protocol [5-minute perfusion] and from the transmigration protocol [30-minute perfusion]) and transmigrated cells (D) was determined in the presence of an anti-VAP-1 mAb (TK-8-14), an SSAO inhibitor (5 μM hydroxylamine), a binding but noninhibiting control mAb (against HLA class I), or vehicle, as appropriate. The results are mean ± SEM of 5 independent assays using different HUVECs and lymphocytes. ^*P < .05; ^**P < .01 (all comparisons between the negative control and the indicated treatment).