Cotreatment with LBH589 and AMN107 causes more depletion of Bcr-Abl in BaF3/Bcr-Abl, BaF3/Bcr-AblT315I, and BaF3/Bcr-AblE255K cells. Cells were treated with the indicated concentrations of LBH589 and/or AMN107 for 24 hours. Following this, the cell lysates were harvested and immunoblotted with the anti–Bcr-Abl antibody. The levels of β-actin served as the loading control.