AMN107 inhibits autophosphorylation of Bcr-Abl and attenuates p-STAT5, p-AKT, c-Myc, and Bcl-xL levels. (A) LAMA-84 cells were treated with the indicated concentrations of AMN107 for 24 hours. Following this, immunoprecipitates were obtained with protein G-agarose beads coated with anti-Abl antibody and immunoblotted with antiphosphotyrosine antibody or anti-Abl antibody. (B) Alternatively, cell lysates of LAMA-84 cells were used for Western blot analyses of p-STAT5, STAT5, c-Myc, Bcl-xL, p-AKT, AKT, and p27, using specific antibodies. The levels of β-actin served as the loading control.