American Society of Hematology

Figure 5.

$Figure 5. Fetal liver HSCs are CD244–. (A) Three-and-nine-tenths percent of fetal liver cells expressed CD244. (B) CD244 expression was detected in only 2.7% of ThylowSca-1+lineage–Mac-1+ cells. CD244, Thy-1, Sca-1, lineage marker, and Mac-1 staining were detected in the FITC, PE-Cy5, APC-Cy7 (PR), PE, and APC channels, respectively. (C) Irradiated recipient mice received transplants of 1950 CD244+ fetal liver cells (blue lines) or 48 050 CD244– fetal liver cells (red lines). All long-term multilineage reconstituting activity was contained within the CD244– cell fraction (5 of 7; the other 2 mice were reconstituted only in the B-cell lineage). None of the mice that received transplants of CD244+ cells (0 of 8) showed any donor cell reconstitution.$

Fetal liver HSCs are CD244^–. (A) Three-and-nine-tenths percent of fetal liver cells expressed CD244. (B) CD244 expression was detected in only 2.7% of ThylowSca-1⁺lineage^–Mac-1⁺ cells. CD244, Thy-1, Sca-1, lineage marker, and Mac-1 staining were detected in the FITC, PE-Cy5, APC-Cy7 (PR), PE, and APC channels, respectively. (C) Irradiated recipient mice received transplants of 1950 CD244⁺ fetal liver cells (blue lines) or 48 050 CD244^– fetal liver cells (red lines). All long-term multilineage reconstituting activity was contained within the CD244^– cell fraction (5 of 7; the other 2 mice were reconstituted only in the B-cell lineage). None of the mice that received transplants of CD244⁺ cells (0 of 8) showed any donor cell reconstitution.

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