American Society of Hematology

Figure 3.

$Figure 3. Fetal liver HSCs are CD150+. (A) Two-and-a-half percent of fetal liver cells expressed CD150. (B) CD150 expression was heterogeneous within the Thylow Sca-1+lineage–Mac-1+ HSC population. For this analysis, CD150, Thy-1, Sca-1, lineage marker, and Mac-1 staining were detected in the FITC, PE-Cy5, APC-Cy7 (PR), PE, and APC channels, respectively. (C) Recipient mice received transplants of 1250 CD150+ fetal liver cells (blue lines) or 48 750 CD150– fetal liver cells (red lines). All multilineage reconstituting activity was contained within the CD150+ cell fraction (10 of 10 mice) not within the CD150– cell fraction (0 of 10 mice). The CD150– cell fraction showed transient B- and T-cell reconstitution (4 of 10 mice) or transient B-lineage reconstitution (3 of 10 mice), but no detectable myeloid lineage reconstitution.$

Fetal liver HSCs are CD150⁺. (A) Two-and-a-half percent of fetal liver cells expressed CD150. (B) CD150 expression was heterogeneous within the Thy^low Sca-1⁺lineage^–Mac-1⁺ HSC population. For this analysis, CD150, Thy-1, Sca-1, lineage marker, and Mac-1 staining were detected in the FITC, PE-Cy5, APC-Cy7 (PR), PE, and APC channels, respectively. (C) Recipient mice received transplants of 1250 CD150⁺ fetal liver cells (blue lines) or 48 750 CD150^– fetal liver cells (red lines). All multilineage reconstituting activity was contained within the CD150⁺ cell fraction (10 of 10 mice) not within the CD150^– cell fraction (0 of 10 mice). The CD150^– cell fraction showed transient B- and T-cell reconstitution (4 of 10 mice) or transient B-lineage reconstitution (3 of 10 mice), but no detectable myeloid lineage reconstitution.

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