Sequence and expression analysis of gng2. (A) Zebrafish Gγ2 protein is 94% identical to mammalian proteins. (B) Phylogram analysis revealed zebrafish Gγ2 protein is highly conserved among vertebrates. The Gγ2 protein contains a CAA (L/S) box (rectangular box, where A was any aliphatic amino acid) at the C-terminus which is conserved among the Gγ2 protein family (right panel in panel B). (C) RT-PCR detection of the maternal gng2 transcript from 1-cell to 128-cell stage and zygotic transcript from high stage onward to 1 dpf. (D-F) gng2 expression in the central nervous system (CNS) and axial vascular tissues at 18-hpf to 24-hpf zebrafish embryos as detected by whole-mount in situ hybridization, insert with higher magnification of the trunk region (F). (G) gng2 expression gradually reduced in the axial vascular tissues but remained in the CNS. (v.m.h. indicates ventral mid- and hindbrain; tel., telecephalon; a.v., axial vasculature; and s.n., spinal cord neurons). All scale bars are 100 μm (D-G).