The mannose receptor CD206 is down-regulated in response to bortezomib. (A) DCs generated from adherent monocytes by a 6-day incubation in the presence of GM-CSF and IL-4 were stimulated for 24 hours with the indicated concentrations of bortezomib. Afterward, cells were washed, stained, and analyzed by flow cytometry. Solid histograms represent the matched isotype controls. (B) DCs generated from adherent monocytes by incubation with GM-CSF and IL-4 were exposed to 1 ng/mL bortezomib for 24 hours. LPS (100 ng/mL), TNF-α (20 ng/mL), CD40L (100 ng/mL), or a cocktail of IL-1 (2 ng/mL), IL-6 (100 ng/mL), PGE₂ (1 μg/mL), and TNF-α (20 ng/mL) was present in the culture medium during the last 16 hours of incubation. Thereafter, cells were harvested, stained, and analyzed by flow cytometry.