Distribution and subcellular localization of Gimap4. (A) Flow cytometric detection of Gimap4. Splenocytes from a wild-type mouse were intracellularly stained with Gimap4-fluorochrome (black), and nonstained cells were used as comparison (white), showing that expression of Gimap4 on lymphocytes is ubiquitous. (B) HeLa cells were transiently transfected with an N-terminal Gimap4-EGFP fusion protein. Cells were incubated with ToPRO3 for nuclear staining (red), and confocal microscopy was used to visualize the localization of Gimap4. HeLa cells transfected with the empty vector served as a control. (C) Different cellular fractions (cytosol, C; membranes, M; nucleus, N) were isolated from splenocytes,27 and Western blot analysis was performed with anti-Gimap4.
