Correlation between G1-HRD-luc and G1-HRD-GFP expression in normal and anemic mice. (A) Bioluminescent images of G1-HRD-luc/G1-HRD-GFP mice treated with PHZ (right) or vehicle (left). Mice were injected intraperitoneally with PHZ or vehicle on days 1 and 2 and imaged on day 5. Areas encircled on the images represent the areas, including the spleen, processed for quantitative analysis. (B) Quantification of the luciferase signal from areas including the spleen, as shown in panel A. Mean signal intensities from PHZ-treated (n = 3) and untreated (n = 3) mice on day 5 are shown. *P < .5 compared with untreated control. (C-E) G1-HRD-luc/G1-HRD-GFP mice treated with PHZ or vehicle were killed on day 5, and spleen cells were examined. (C) GFP intensities of splenic mononuclear cells prepared from PHZ-treated (right panel) and untreated (left panel) mice. (D) Luciferase activities of whole-cell extracts from splenic mononuclear cells in GFP^–, GFP^low, and GFP^highfractions. Each bar represents the average luciferase activity divided by the cell number in PHZ-treated (▦, n = 3) and untreated (□,n = 3) cells. Error bars indicate SD. (E) Luciferase activities in GFP^–, EEP, and LEP fractions from untreated splenic mononuclear cells.