Figure 3.
Triptolide reduced the XIAP level and induced caspase-dependent cell death in leukemic cells. OCI-AML3 cells at a density of 0.2 × 106/mL were treated for 24 hours with various concentrations of triptolide in the absence or presence of the general caspase inhibitor IDN-1965 (20 μM). (A) Caspase-3 activation and PARP cleavage were analyzed by Western blot. (B) Cell viability was determined by annexin V staining with PI. (C) XIAP levels were analyzed by Western blot. (D) U937 and HL60 cells at a density of 0.2 × 106/mL and (E) AML blasts (n = 6) at a density of 1 × 106/mL were treated for 24 hours with various concentrations of triptolide. XIAP and caspase-3 protein levels were determined by Western blot.
