Figure 2.
Triptolide induced significant cell death in AML samples. (A) Comparison of triptolide-induced cell death in AML samples with that in normal bone marrow samples. Cells from 11 AML blasts and 3 normal bone marrow samples at a density of 1 × 106/mL were treated with various concentrations of triptolide. Cell death was determined by annexin V staining with PI after 24 hours of treatment. Normal bone marrows were stained with CD34-APC after triptolide treatment, and PS/annexin V was determined in the CD34+ population. (B) Results of colony-formation assays in blasts from 5 AML samples and cells from 3 normal bone marrow samples treated with the indicated concentrations of triptolide.
