Differential expression of proteins associated with cell proliferation using 2D analysis. (Ai,iv,vii) Control fibroblasts; (ii,v,viii) pretreatment fibroblasts; (iii,vi,ix) posttreatment fibroblasts. These detailed pictures of the silver-stained gels demonstrate that protein expression pattern in the patient's fibroblasts after treatment mirrors that observed in control fibroblasts. Arrows identify the following proteins: (1) Cu/Zn SOD; (2) Mn SOD; (3) Profilin I; and (4) MIF. (B) Effect of the different drugs used in the conditioning procedure on the expression of MnSOD (arrowheads) in the patient fibroblasts. All drugs increased the expression of MnSOD in the patient to levels similar to control fibroblasts. (i) Control fibroblasts; (ii) patient fibroblasts untreated; (iii-v) patient fibroblasts treated with BCNU (iii), Ara-C (iv), or melphalan (v). All images were acquired with a GS-800 scanner (BioRad, Palo Alto, CA) and processed with Adobe Photoshop 7.0 (Barcelona, Spain).