KSHV infection of CD34⁺HPCs in the absence of polybrene. CD34⁺ HPCs were infected with KSHV in the presence or absence of polybrene. CD34⁺ HPCs infected with KSHV were permeabilized and incubated with primary rat monoclonal antibody (mAb) directed against KSHV LANA-1 followed by incubation with AMCA-labeled rabbit anti-rat IgG secondary antibody. Samples were analyzed by flow cytometry for the presence of LANA-1, and cells were gated according to patterns demonstrated by staining with secondary antibody alone.