Increase in CD11b exposure on neutrophils with different FcγRIIa/FcγRIIb2 mRNA ratio upon stimulation with polymeric IgG. (A) Histograms showing the up-regulation of CD11b on neutrophils stimulated with various concentrations of polymeric IgG. Representative examples of each group are shown. (B) Induction of CD11b accumulation in whole blood, obtained from several individuals selected from the white population, after stimulation with either 50 μg/mL dimeric IgG in the presence or absence of blocking Fab anti-FcγRII and/or blocking F(ab′)2 anti-FcγRIII or fMLP supplemented with cytochalasin B. Error bars represent SEM. (C) Whole blood, obtained from several individuals selected from the white population, was stimulated with 50 μg/mL dimeric IgG, and induction of CD11b accumulation was measured. The difference in exposure between the 3 groups of neutrophils tested was significant.
