Induction of CD25 in monocyte-derived DCs. (A) Analysis of cell-surface expression of CD25 by the different DC populations using flow cytometry. Three to 12 experiments were performed for each of the conditions; a representative experiment for each condition is shown. Isotype controls are shown as gray area underneath the dotted line; specific antibodies are reflected by the black lines. (B) Kinetics of surface CD25 expression on mo-DCs, matured with and without PGE2. Isotype controls are shown as gray area underneath the dotted line; specific antibodies are reflected by the black lines. One representative experiment of 2 is shown. (C) Soluble CD25 was assessed by ELISA in cell culture supernatants from different DC populations. Three to 12 experiments were performed for each of the conditions. Black bars represent PGE2-treated DCs; mean ± standard deviation of all experiments is shown. ^*P < .05. (D) Kinetics of sCD25 production by mature mo-DCs under the influence of PGE2, as shown for 2 independent donors. ○ represents immature DCs; • DCs matured with TNFα and αCD40; and , DCs matured with TNFα and αCD40 in the presence of PGE2.