CD34⁺ FLC infusion improves in vivo functions of human T cells in NOD/SCID mice that received a graft of human fetal Thy/Liv. (A) Skin xenograft survival in NOD/SCID control mice (n = 5), NOD/SCID mice that received human Thy/Liv (n = 5), and NOD/SCID mice that received human Thy/Liv/CD34⁺ FLCs (n = 6). (B) Fluorescence microscopy showing infiltration by human CD3⁺ T cells in representative porcine skin grafts from NOD/SCID mice that received a transplant of Thy/Liv and Thy/Liv/CD34⁺ FLCs. Normal porcine skin was used as staining control. (C) Splenocytes were prepared from NOD/SCID control mice that did not undergo transplantation (n = 5), NOD/SCID mice that received Thy/Liv (n = 5), and NOD/SCID mice that received Thy/Liv/CD34⁺ FLCs (n = 6) 120 days after skin grafting and analyzed for xenogeneic (antipig) and allogeneic (antihuman) MLR. Data are mean ± SEMs. *P < .05; **P < .01.