Figure 2.
Figure 2. Measurement of NO levels during inhibition of eNOS in endothelial cells incubated with hydroxyurea (HU). (A) Hydroxyurea (20 μM) induction of NO is inhibited by competitive eNOS inhibitor NG-nitro-l-arginine (0.1 mM) in HUVECs in the presence of supplementary 0.1 mM l-arginine (*P < .05 versus cells treated with hydroxyurea and NG-nitro-l-arginine). (B) L-NAME (0.1 mM) also inhibited hydroxyurea (20 μM) induction of NO production in TrHBMECs in the presence of supplementary 0.1 mM l-arginine (*P < .05 versus cells treated with hydroxyurea and L-NAME). Values represent mean ± SEM (n = 3).

Measurement of NO levels during inhibition of eNOS in endothelial cells incubated with hydroxyurea (HU). (A) Hydroxyurea (20 μM) induction of NO is inhibited by competitive eNOS inhibitor NG-nitro-l-arginine (0.1 mM) in HUVECs in the presence of supplementary 0.1 mM l-arginine (*P < .05 versus cells treated with hydroxyurea and NG-nitro-l-arginine). (B) L-NAME (0.1 mM) also inhibited hydroxyurea (20 μM) induction of NO production in TrHBMECs in the presence of supplementary 0.1 mM l-arginine (*P < .05 versus cells treated with hydroxyurea and L-NAME). Values represent mean ± SEM (n = 3).

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