CXCL13 is produced by TECs and is overproduced by TECs from MG patients. (A) Determination by ELISA of CXCL13 concentration in the culture supernatants of subcultured TECs (at days 2, 4, and 7), thymocytes, MITCs, and thymic fibroblasts. Each histogram represents the mean value of 3 different experiments and SEM. (B) CXCL13 gene amplification by real-time RT-PCR on RNAs isolated from TECs obtained at days 3, 6, and 7 of primary cultures and days 2, 4, and 7 of subcultures. Each histogram represents the mean value and SEM of 2 different experiments. (C) Amplification by real-time PCR of CXCL13 gene on RNAs isolated from TECs prepared from 4 patient thymuses (MG1-4), 1 adult control (A ctrl), and 3 newborn controls (N ctrl1-3). Each histogram represents the mean and SEM of 2 different experiments.