Cytokine production by T cells activated with ^HGFMo. (A) Supernatants of primary MLR cultures containing cytokine-differentiated DCs (indicated in the x-axis) and CD4⁺CD25^- T cells were used for the measurement of prototypic Th1-, Th2-, and Tr1-related cytokines. Data represent mean ± SD recorded in 6 independent experiments performed in duplicate. ^*P < .01 compared with MLR cultures performed with GM4-DCs. (B) IL-10 levels were measured in the supernatant of secondary MLR cultures performed with T cells recovered from the primary MLR and same-donor, cytokine-differentiated DCs (indicated in the x-axis). ^*P < .01 compared with secondary MLR cultures performed with GM4-DCs. (C) Cells recovered from primary MLR cultures established with ^HGFMo (1:3 stimulator-to-responder ratio) were counterstained with PE-conjugated anti-CD4 or anti-CD86 mAbs, fixed/permeabilized as detailed in “Materials and methods,” and labeled with FITC-conjugated anti-IL-10 mAbs. The percentage of IL-10⁺ cells is indicated in each histogram. One representative experiment of 3 with similar results is shown.