Phenotypic features of cytokine-differentiated monocytes. DCs were generated as detailed in “Materials and methods.” (A) Phenotypic profile of monocytes cultured with GM-CSF + IL-4 (GM4-DCs) or with HGF (^HGFMo). Numbers indicate MFI of mAb staining measured in a representative experiment of 9 with similar results. (B) Endocytic capacity of ^HGFMo. DCs were either left immature or matured with TNF-α for 48 hours and were then incubated for 1 hour with FITC-dextran at 37°C. Control cultures were established at 4°C to measure background fluorescence.²⁶ Numbers indicate MFI of mAb staining measured in a representative experiment of 4 with similar results. (C) Expression of informative DC Ags on monocytes cultured with GM-CSF, IL-4, and HGF, with GM-CSF and IL-4, or with HGF alone. Numbers indicate MFI of mAb staining measured in a representative experiment of 4 with similar results.