In vivo assay of RANTES-induced leukocyte recruitment into murine air pouches. The air pouches were initially treated with PBS, 20 μM CGS21680, or 30 μM ZM241385 together with 20 μM CGS21680 for 30 minutes, then stimulated with either 1 μg/mL RANTES, 20 μM CGS21680, 3 μM CPA, or the combination of CGS21680 and 30 μM ZM241385, for 4 hours. The cells were then harvested in PBS supplemented with EDTA and heparin, stained, and counted. (This graph represents data from one of 3 in vivo experiments, each bar represents 4 mice; ^*P < .01.)