Hp expression in myeloid 32Dcl3 cells is induced by C/EBPϵ. Myeloid 32Dcl3 cells constitutively expressing a fusion protein containing the C/EBPϵ wild-type and the ligand-binding domain of the estrogen receptor (32Dcl3-C/EBPϵ-ER) were induced with 4-HT, resulting in nuclear translocation of C/EBPϵ-ER. Hp mRNA levels were measured relative to β-actin levels by reverse transcription (RT) real-time PCR at the indicated time points. Changes of Hp mRNA levels at indicated time points after 4-HT induction were calculated relative to the Hp mRNA level before 4-HT induction (0 hour) (mean ± SD; n = 3). Myeloid 32Dcl3 cells constitutively expressing the ligand-binding domain of the estrogen receptor (32Dcl3-ER) served as control.