Hp and lactoferrin are absent in blood cells from a patient with SGD. Blood samples from a patient with SGD and a healthy donor (control) were depleted of erythrocytes, and lysates including total nuclear cells (neutrophils and mononuclear cells) and residual plasma were subjected to Western blot analyses using rabbit anti-human MPO, Lf, and Hp antibodies. Western blot analyses demonstrated that blood cells from the SGD patient expressed the azurophil granule protein MPO at comparable levels but expressed neither the specific granule protein Lf nor the highly-glycosylated Hp β-chain with a molecular weight of 45 to 65 kDa. Contamination of cell lysates with residual plasma resulted in the detection of Hp β-chain with a low molecular weight (39 kDa) in the control sample and the sample obtained from the SGD patient.